Abstract: In order to analyze the type and concentration of human volatile odorant molecules (human VOCs), this study developed a silicon nanowire (SiNW) array electronic nose based on mosquito odorant binding proteins (OBPs) modified to achieve real-time and highly sensitive detection to human VOCs. The OBPs were modified on the surface of SiNW to recognise human VOCs, which caused the change of OBPs' conformation, and then the change of electric field on the SiNW surface. Finally, the device recorded all the current changes and achieved the real-time detection and analysis of human VOCs. Importantly, through circular dichroism, the conformation changes caused by the combination of VOCs and OBPs were detected, which proved the biochemical feasibility of constructing artificial electronic nose. The electronic nose designed in this study has the characteristics of high sensitivity and selectivity. It can detect human VOCs molecules at 5ppb and different types of modified OBPs have obvious selectivity for human VOCs. SiNW array devices are highly compatible with complementary metal oxide semiconductor(CMOS) and can be prepared in batches using top-down manufacturing methods. This study develops customizable human odorant electronic noses for the future and provides an important biochemical and device foundation.