Abstract: Aldehyde groups were produced on silica surface through silanisation using 3-Aminopropyltriethoxysilane and Terephthal-dicarboxaldehyde self-assembly.125nm ramification was formed on silica surface after modification process.Immunoglobulin G（IgG） was immobilized on silica surface through condensation reaction between an amine and an aldehyde.Activity of IgG was investigated through antigen-antibody reaction.A micro-fluidics chip with an elliptical micro-pillar array was fabricated.The inner surface area and contact opportunity of fluid increased than the chip without micro-pillar array.Human IgG was immobilized on the micro-pillar arrays through such a process.Then,fluoroisothiocyanate（FITC）-conjugated goat antibody to human IgG phosphate buffer solution（PBS） solution was introduced into the channel of the micro-fluidics chip.The results indicate that the fluorescence intensity increases up to 15 times after going through the micro-fluidics chip.Experiment show antibody can be attached on silica surface though this way and its activity is retentive.Protein can be enriched through such a surface modified micro-fluidics chip.