Abstract: Cell membrane mimetic phosphorylcholine（PC） groups were grafted onto cover glass surfaces via three new routes using phosphorylcholine dichloride（PC-2Cl）,chlorodimethyl-n-octadecylsilane（C₁₈） and 1,6-diisocyanatohexane（HDI） as the reagents.Route Ⅰ,the surface-silanol group of the glass was reacted directly with PC-2Cl.Route Ⅱ,the surface-silanol group was reacted with HDI,and then the resulted end-amine group after hydrolysis was reacted with PC-2Cl..Route Ⅲ,C 18 was grafted grafted first,and then followed with the procedures of route Ⅱ.The effects of surface modification were evaluated through the measurements of dynamic contact angle（DCA） and X-ray photoelectron spectroscopy（XPS）.The results indicated that,the three routes in this paper could graft PC groups onto the surfaces of cover glasses successfully,while route Ⅱ or Ⅲ could obtain higher PC coverage on the surface and tunable hydrophilicity.