Abstract: Based on the mechanism of capture, detection and release, this paper designs and produces a chip for capturing and releasing single cells by using the three-layer microfluidic chip technology of the valve on the integrated chip. COMSOL simulation is used to optimize the chip structure to increase the capture rate of cells. The performance of the valve in different Polydimethylsiloxane(PDMS) combination and different sample loading pressures are compared. And the ability of the valve to operate for a long time is verified. The effectiveness of the capture and release mechanism of the chip is tested and the process of capture, detection and release can be realized. The fabrication process of the chip is simple, and the yield can reach more than 95%. This method of capturing and detecting before releasing provides a new technical approach for single cell separation and collection.