Abstract: Giant magnetoimpedance （GMI） effect was investigated in the frequency range of 100kHz～40MHz in multilayered FeSiB/Cu/FeSiB with different thickness of FeSiB films. With magnetic field H_a applied along the longitudinal direction of the sample, the GMI ratio increases with H_a, reaching a posttive maximum value at a certain field and then decreases to the negative GMI ratio with further increase of H_a. At a frequency of 3.2MHz, the positive maximum GMI ratio is 13.5% for H_a = 2.4kA/m, and a larger negative GMI ratio,-9.2% is obtained for H_a = 9.6kA/m in multilayered FeSiB/Cu/FeSiB films which FeSiB film is 1.8μm thick. While, for multilayered FeSiB/Cu/FeSiB films which FeSiB film is 1.0μm thick, the maximum GMI ratio of 5.8% is obtain at 1.6kA/m for a frequency of 40MHz. In addition, the multilayered films displayed large negative GMI ratio with magnetic field applied along the transverse direction of the samples, and a negative GMI ratio, -12% is obtained at 5.2MHz for H_a= 9.6kA/m in multilayered FeSiB/Cu/FeSiB films with FeSiB film thickness of 1.8μm. The large negative GMI ratio and the positive maximum CMI ratio are correlated to the easy axis orientation and the thickness of FeSiB film in the multilayered FeSiB/Cu/FeSiB films.