PCR微池芯片的设计、制作及实现样品扩增

Design, fabrication of micro-chamber PCR chip and realization of sample amplification

摘要: 设计并制作了一种石英玻璃材料的PCR(PolymeraseChainReaction)微池芯片,使用商用热循环仪控温。通过实时测量芯片微池内温度,改进温度控制程序,使用BSA(牛血清白蛋白)处理微池内壁等优化工作,实现了DNA片段的有效PCR扩增。

Abstract: A kind of Micro-chamber PCR Chip was designed and fabricated. The material of the chip is SiO₂ glass. Commercial thermal cycler was used to control the temperature. The thermal control procedure was optimzied through real-time temperature measure inside the chip chamber. The chamber wall was covered by BSA （Bovine Serum Albumin）. PCR amplification of DNA fragment is realized.