Abstract: Based on single-atom catalysis the greatest atom utilization, the regulation of electronic structure, regulation of highly active sites, special performance of catalytic reaction, and the characteristics of high stability, which will be expanded in the field of electrochemical biosensor materials modification. Researchers further explore single-atom catalyst application in the field of electrochemical biosensing. In this paper, N doped carbon-based materials as a carrier, metal coordination unit as a model, researchers studied the carrier metal coordination unit of single atomic catalytic activity of the catalyst, the influence of metal coordination-N doped carbon-based materials and the latest research progress in the field of electrochemical biosensing applications, summarized the heterogeneous catalyst application prospect and challenges in the field of electrochemical biosensing applications.